Genotyping of Candida albicans Isolates Obtained from Vulvovaginal Candidiasis Patients from Zanjan, Iran, Based on ABC and RPS Typing Systems

Background and Purpose: Genotyping of pathogenic microorganisms is important for epidemiological studies the adoption appropriate strategies to control infectious diseases. In this regard, present study aimed genotype Candida albicans strains isolated from vulvovaginal candidiasis (VVC) patients using combined ABC type (25SrDNA) repetitive sequence (RPS) typing systems. systems (RPS). Materials Methods: total, 140 with VVC were investigated. Vaginal discharges collected on Sabouraud dextrose agar identified by CHROMagar. After species identification, a polymerase chain reaction system targeting 25S rDNA as well ALT repeats in RPS was designed determine C. genotypes. The dendrogram constructed zero-one matrix data based combination Statistical analysis performed SPSS software (version 23). Results: 41 (29.3%) isolates obtained patients. most common that included glabrata (56.1%) (39%). Genotype A3 five (31.25%) had highest frequency, followed B2/3 three (18.3%), A3/4, C3/4, B3/4 two (12.5%), C2/3 C3 one isolate (6.25%), respectively. No significant association found between genotypes antifungal resistance (P<0.05). Conclusion: results showed non-albicans are more prevalent patients, compared albicans. also indicated typings useful rapid genotyping differentiation regional small-scale studies.